This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison
Isoforms of human CDH23 precursor protein
Displayed below are 6 cdh23 precursor protein isoforms. These isoforms are all expressed in the wildtype human, and isoforms result from alternative splicing of the mRNA. This does not imply that each isoform can lead to a fully functional cdh23 protein. It only indicates that in vivo cdh23 is generally functional, and does not lead to deafness.
It would be very interesting in the future to test whether or not each isoform can give rise to functional cdh23 by themselves, or if one isoform predominates the production of functional cdh23.
It would be very interesting in the future to test whether or not each isoform can give rise to functional cdh23 by themselves, or if one isoform predominates the production of functional cdh23.
Isoforms 2-4 only have sequence knockouts, whereas 5 and 6 have both knockouts and sequence alterations (Uniport)
Trypsin cuts cadherin 23
in digestion for consequent proteomic analysis, trypsin cuts right of the following positions:
27 76 90 95 98 116 117 140 184 191 212 256 265 269 301 315 323 408 412 414 426 445 447 505 520 528 536 546 548 563 571 582 584 700 702 720 724 736 739 747 758 779 822 832 834 842 854 855 869 928 935 955 957 964 975 1010 1013 1042 1048 1053 1060 1080 1081 1125 1136 1140 1151 1161 1168 1170 1189 1193 1215 1219 1236 1247 1255 1276 1334 1349 1359 1363 1379 1381 1397 1403 1417 1437 1442 1487 1502 1507 1508 1509 1551 1555 1572 1577 1580 1594 1596 1684 1687 1696 1706 1723 1746 1762 1771 1802 1804 1805 1811 1824 1826 1840 1885 1895 1897 1916 1918 1923 1929 1936 1939 1940 1961 2022 2029 2107 2115 2126 2134 2141 2149 2206 2217 2222 2243 2248 2270 2278 2304 2325 2353 2358 2373 2382 2447 2465 2467 2468 2478 2488 2489 2576 2623 2629 2649 2654 2659 2678 2681 2727 2743 2775 2789 2793 2795 2804 2809 2815 2833 2845 2848 2860 2892 2915 2935 2952 2956 2958 2967 2969 2998 2999 3001 3016 3020 3026 3035 3039 3043 3057 3091 3095 3096 3097 3099 3109 3122 3138 3168 3175 3186 3189 3200 3206 3211 3216 3224 3226 3227 3232 3236 3267 3269 3277 3306 3310 3325 3328 3333 3339 3341
27 76 90 95 98 116 117 140 184 191 212 256 265 269 301 315 323 408 412 414 426 445 447 505 520 528 536 546 548 563 571 582 584 700 702 720 724 736 739 747 758 779 822 832 834 842 854 855 869 928 935 955 957 964 975 1010 1013 1042 1048 1053 1060 1080 1081 1125 1136 1140 1151 1161 1168 1170 1189 1193 1215 1219 1236 1247 1255 1276 1334 1349 1359 1363 1379 1381 1397 1403 1417 1437 1442 1487 1502 1507 1508 1509 1551 1555 1572 1577 1580 1594 1596 1684 1687 1696 1706 1723 1746 1762 1771 1802 1804 1805 1811 1824 1826 1840 1885 1895 1897 1916 1918 1923 1929 1936 1939 1940 1961 2022 2029 2107 2115 2126 2134 2141 2149 2206 2217 2222 2243 2248 2270 2278 2304 2325 2353 2358 2373 2382 2447 2465 2467 2468 2478 2488 2489 2576 2623 2629 2649 2654 2659 2678 2681 2727 2743 2775 2789 2793 2795 2804 2809 2815 2833 2845 2848 2860 2892 2915 2935 2952 2956 2958 2967 2969 2998 2999 3001 3016 3020 3026 3035 3039 3043 3057 3091 3095 3096 3097 3099 3109 3122 3138 3168 3175 3186 3189 3200 3206 3211 3216 3224 3226 3227 3232 3236 3267 3269 3277 3306 3310 3325 3328 3333 3339 3341
the 3000-3100 range shows definate coil-coil presence in cdh23. A coil coil protein is one in which two identical amino acid segments wrap around each other. (http://cis.poly.edu/~jps/coilcoil.html)
References:
Uniport Cadherin 23 protein isoforms: http://www.uniprot.org/uniprot/Q9H251#Q9H251-1
http://cis.poly.edu/~jps/coilcoil.html
Uniport Cadherin 23 protein isoforms: http://www.uniprot.org/uniprot/Q9H251#Q9H251-1
http://cis.poly.edu/~jps/coilcoil.html
Ben Hofeld, [email protected], last updated: 5.15.2010, Link to course page:www.gen677.weebly.com
